How to get antisense sequence from any strings ?

[galaxy001]

How to make a new Read object from string, so that I can call Read.antisense ?

fa = pyfastx.Fastx("x.fq")
for name,seq,_ in fa:
   seqF = seq[:100]

I want to get antisense of seqF.
However, fa[-1][:100] not working as "TypeError: 'Read' object is not subscriptable".

[lmdu]

Currently, the Read object of pyfastx could not support for slicing. You can implement a function for conversion like this:

def reverse_complement(s):
    bases = {'A': 'T', 'G': 'C', 'T': 'A', 'C': 'G', 'N': 'N'}
    return ''.join([bases[b] for b in s][::-1])

fa = pyfastx.Fastx("x.fq")
for name, seq, _ in fa:
   seqF = reverse_complement(seq[:100])